Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

BBA Gene Regulatory Mechanisms includes reports that describe novel insights into mechanisms of transcriptional, post-transcriptional and translational gene regulation. Special emphasis is placed on papers that identify epigenetic mechanisms of gene regulation, including chromatin, modification, and remodeling. This section also encompasses mechanistic studies of regulatory proteins and protein complexes; regulatory or mechanistic aspects of RNA processing; regulation of expression by small RNAs; genomic analysis of gene expression patterns; and modeling of gene regulatory pathways. Papers describing gene promoters, enhancers, silencers or other regulatory DNA regions must incorporate significant functions studies.The journal does not favorably review manuscripts identifying a miRNA-target pair without additional insights into the repression mechanism or significant advances in understanding regulatory pathways. In addition, the following elements should be an integral part of the study:?In silico prediction of miRNA targets must be experimentally verified using appropriate luciferase constructs and assays;?To exclude non-functional miRNA/mRNA interactions a reporter system including the whole 3'UTR of the target gene downstream the "luciferase" or GFP should be considered;?Any miRNA modulation should be validated by measuring the expression of the putative protein

BBA基因调控机制包括对转录、转录后和翻译基因调控机制的新见解的报告。特别强调的论文，以确定表观遗传机制的基因调控，包括染色质，修改和重塑。本节还包括调控蛋白和蛋白复合物的机制研究;RNA处理的调控或机制方面;小rna对表达的调控基因表达模式的基因组分析;以及基因调控通路的建模。描述基因启动子、增强子、沉默子或其他调节DNA区域的论文必须包含重要的功能研究。如果没有对抑制机制的深入研究或在理解调控途径方面的重大进展，该杂志不会对鉴定mirna -靶点对的手稿进行正面评价。此外，下列要素应成为研究的一个组成部分:?在miRNA目标的硅预测中，必须使用合适的荧光素酶构建物和检测方法进行实验验证;?为了排除非功能性miRNA/mRNA相互作用，应该考虑包括“荧光素酶”或GFP下游目标基因的整个3’utr的报告系统;?任何miRNA的调节都应该通过测量假定蛋白的表达来验证