磁铁矿作用下DvH阴极生物膜的响应及转录组分析

来源：中国环境科学杂志2024年第11期北京时间：

作者：滕敏, 朱曦, 曾翠平, 胡佳萍, 刘广立, 骆海萍

单位：1. 中山大学环境科学与工程学院, 广东省环境污染控制与修复技术重点实验室, 广东 广州 510006;2. 深圳合成生物学创新研究院, 中国科学院深圳先进技术研究院, 广东 深圳 518055

摘要：This study investigated the response mechanism of the biofilm by constructing a Desulfovibrio vulgaris Hildenborough (DvH) biocathode, under the influence of magnetite nanoparticles (MNPs). The results showed that the conversion rate of SO42- to S2- of the biocathode mediated by MNPs increased from 6.8% to 37.9%, compared to the group without MNPs. The results of the linear sweep voltammetry test revealed that, within the potential range of 0~-0.81V, the cathodic current observed in the group with MNPs was higher than that of the control group, suggesting that MNPs enhance the electrocatalytic activity of the cathodic biofilm. The comparative transcriptome analysis of DvH cathodic biofilm revealed that MNPs can facilitate hydrogen utilization in DvH by upregulating genes encoding [FeFe] hydrogenase, Hmc, and ATP synthase. In addition, genes encoding sulfate adenylyltransferase and adenylylsulphate reductase, both crucial enzymes in sulfur metabolism, were upregulated with MNPs addition, which contributed to an improved conversion rate of SO42--S2-. MNPs upregulated genes related to Flp/Tad pili assembly and PEP-CTERM protein, thereby augmenting the adhesion of DvH and promoting biofilm formation on the cathode. These findings contribute to a novel theoretical framework for the development of highly efficient microbial cathodic electrochemical systems.

关键词：微生物电化学系统,硫酸盐还原菌,生物阴极,纳米磁铁矿,转录组学

基金资助：国家自然科学基金资助项目(42077286);广东省自然科学基金资助项目(2023A1515011846)